Coding

Part:BBa_K3819147:Design

Designed by: Yujiao Peng   Group: iGEM21_UESTC-China   (2021-09-25)

sdbA, encoding a protein which specifically binds the dockerin domain of CipA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized to improve expression in yeast and the restriction site optimized to connect a linker directly.


Source

Hungateiclostridium thermocellum naturally expressed the entire anchor scaffold (SdbA), whose GenBank number was U49980.1. An immunofluorescence tag should also be added to the C-terminal of SdbA gene, and flag-Tag should be selected, consisting of 8 amino acid residues, N-DykDDDDK-C (1012 Da).

References